Lymphocyte regulation and tolerance
UMRS 1064/ITUN-Centre de Recherche en Transplantation et en Immunologie
The team 4 of CRTI addresses questions related to T and B lymphocytes in transplantation and in multiple sclerosis (MS), with a basic and translational approach, thanks to the bio-collections and databases. Using state-of art single immune cell analysis, we are defining the phenotype of these lymphocyte populations from MS patients to better delineate their specificities and pathogenic role. As recent data suggest an important role of focal inflammation and reactive glia in white matter autoimmune pathology, we are applying LCM to white matter sections to better analyze the molecular profile of in situ reactive astrocytes in the context of MS and its animal models.
Immune cells (CD45high, red) attracted by CCL2 expressing white matter astrocytes (green). From PMID 20404154.
Key words: Lymphocytes, Glia, multiple sclerosis, neuroinflammation, steroids, FACS, Laser Capture Microdissection
Collaborations that have resulted in joint publications with other teams of the network:David VAUDRY / PRIMACEN
Ongoing collaborations which have not yet conducted to joint publications:Thierry CHARLIER / IRSET INSERM U1085/ Unversité de Rennes 1
Main publications over the last few years:
. Neuropathologic, phenotypic and functional analyses of Mucosal Associated Invariant T cells in Multiple Sclerosis. Clin Immunol 2016, 166: 1-11.
. Involvement of CD8(+) T Cells in Multiple Sclerosis. Front Immunol 2015, 6: 604.
. Targeting the CD80/CD86 costimulatory pathway with CTLA4-Ig directs microglia toward a repair phenotype and promotes axonal outgrowth. Glia 2015, 63: 2298-312.
. Transcript analysis of laser capture microdissected white matter astrocytes and higher phenol sulfotransferase 1A1 expression during autoimmune neuroinflammation. J Neuroinflammation 2015, 12: 130.
. Oral versus intravenous high-dose methylprednisolone for treatment of relapses in patients with multiple sclerosis (COPOUSEP): a randomised, controlled, double-blind, non-inferiority trial. Lancet 2015, 386: 974-81.
. Expanded CD8 T-cell sharing between periphery and CNS in multiple sclerosis. Ann Clin Transl Neurol 2015, 2: 609-22.
. Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis. J Immunol Res 2015, 2015: 673503.
. The autoimmune concept of multiple sclerosis. Presse Med 2015, 44: e103-12.
. Sex steroids and neuroprotection in spinal cord injury: a review of preclinical investigations. Exp Neurol 2014, 259: 28-37.
. Exacerbation of experimental autoimmune encephalomyelitis in prion protein (PrPc)-null mice: evidence for a critical role of the central nervous system. J Neuroinflammation 2012, 9: 25.
. NOV/CCN3 upregulates CCL2 and CXCL1 expression in astrocytes through beta1 and beta5 integrins. Glia 2010, 58: 1510-21.
. Reduced expression of plasma membrane calcium ATPase 2 and collapsin response mediator protein 1 promotes death of spinal cord neurons. Cell Death Differ 2010, 17: 1501-10.
. Estradiol inhibits ongoing autoimmune neuroinflammation and NFkappaB-dependent CCL2 expression in reactive astrocytes. Proc Natl Acad Sci U S A 2010, 107: 8416-21.
. NOV/CCN3 promotes maturation of cerebellar granule neuron precursors. Mol Cell Neurosci 2010, 43: 60-71.