|Réseau LARC - Neurosciences||
Plate-forme de Protéomique
Université de Rouen
The Proteomic Platform (PFP) includes 3 facilities: electrophoresis, mass spectrometry and peptide sequencing. The PfP has different equipment: robots, mass spectrometers, microsequencers, nano-LC, ... to meet user demands in the field of proteomic. The PfP is developing methodological approaches for the analysis of biological processes such as protein phosphorylation. The PfP contributes to the training of permanent staff and students. A quality management system has been established and operates under ISO 9001 version 2000 requirements.
Main publications over the last few years:
. Consequences of acclimation on the resistance to acute thermal stress: Proteomic focus on mussels from pristine site. Mar Environ Res 2016, 121: 64-73.
. Gut Commensal E. coli Proteins Activate Host Satiety Pathways following Nutrient-Induced Bacterial Growth. Cell Metab 2016, 23: 324-34.
. Biochemical Characterization of Human Anti-Hepatitis B Monoclonal Antibody Produced in the Microalgae Phaeodactylum tricornutum. PLoS One 2015, 10: e0139282.
. Development of 'Redox Arrays' for identifying novel glutathionylated proteins in the secretome. Sci Rep 2015, 5: 14630.
. Enteral delivery of proteins enhances the expression of proteins involved in the cytoskeleton and protein biosynthesis in human duodenal mucosa. Am J Clin Nutr 2015, 102: 359-67.
. A novel mechanism of autophagic cell death in dystrophic muscle regulated by P2RX7 receptor large-pore formation and HSP90. Autophagy 2015, 11: 113-30.
. Glutamine enema regulates colonic ubiquitinated proteins but not proteasome activities during TNBS-induced colitis leading to increased mitochondrial activity. Proteomics 2015, 15: 2198-210.
. Biochemical composition of haemagglutinin-based influenza virus-like particle vaccine produced by transient expression in tobacco plants. Plant Biotechnol J 2015, 13: 717-25.
. Bacterial ClpB heat-shock protein, an antigen-mimetic of the anorexigenic peptide α-MSH, at the origin of eating disorders. Transl Psychiatry 2014, 4: e458. doi.
. Linkage of inflammation and oxidative stress via release of glutathionylated peroxiredoxin-2, which acts as a danger signal. Proc Natl Acad Sci U S A 2014, 111: 12157-62.
. Enteral glutamine infusion modulates ubiquitination of heat shock proteins, Grp-75 and Apg-2, in the human duodenal mucosa. Amino Acids 2014, 46: 1059-67.
. Comparison of protein-extraction methods for gills of the shore crab, Carcinus maenas (L.), and application to 2DE. J Biomol Tech 2013, 24: 218-23.
. Evaluation of ubiquitinated proteins by proteomics reveals the role of the ubiquitin proteasome system in the regulation of Grp75 and Grp78 chaperone proteins during intestinal inflammation. Proteomics 2013, 13: 3284-92.
. An enteral leucine supply modulates human duodenal mucosal proteome and decreases the expression of enzymes involved in fatty acid beta-oxidation. J Proteomics 2013, 78: 535-44.
. Rational design of a low molecular weight, stable, potent, and long-lasting GPR103 aza-β3-pseudopeptide agonist. J Med Chem 2012, 55: 7516-24.
. Impact of toxicant exposure on the proteomic response to intertidal condition in Mytilus edulis. Comp Biochem Physiol Part D Genomics Proteomics 2011, 6: 357-69.
. Effects of an enteral glucose supply on protein synthesis, proteolytic pathways, and proteome in human duodenal mucosa. Am J Clin Nutr 2011, 94: 784-94. doi.
. Involvement of stathmin 1 in the neurotrophic effects of PACAP in PC12 cells. J Neurochem 2010, 114: 1498-510.